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World's first, autologous iPS cell bank
Our clinic has newly begun providing iPS cell production and storage services for individuals. iPS cells are induced pluripotent stem cells developed by Professor Shinya Yamanaka, current director emeritus of the iPS Cell Research Institute at Kyoto University, and are cells that have acquired pluripotency and self-renewal ability by introducing four types of genes into somatic cells. We are partnering with ICE, Inc., which holds patents on iPS cell production and culture technology, to secure individual health resources and provide preparation for future regenerative medicine and personalized medicine.
What are iPS cells?
Pluripotent stem cells have the ability to reprogram differentiated cells by introducing four genes (Oct3/4, Sox2, Klf4, c-Myc) into somatic cells such as skin, and differentiate into various cells. The roles of the four genes are shown below.
Oct3/4: A transcription factor involved in cell reprogramming and plays an important role in maintaining the undifferentiated state of ES cells and iPS cells.
Sox2: A transcription factor involved in cell reprogramming and maintenance of pluripotency, and is said to be involved in the maintenance of cancer stem cells and tumor formation.
Klf4: A transcription factor that controls cell differentiation, proliferation, and apoptosis and regulates the cell cycle. Works to suppress cancer.
c-Myc: A gene that promotes cell proliferation, differentiation, and tumorigenesis, and is an important transcription factor for efficient production of iPS cells.
The problem with iPS cells is that when they are created by integrating genes into the host cell’s DNA, the DNA
There has been talk of concerns that this damage could lead to carcinogenicity, but we are
Adopts a method that uses the Sendai virus vector, an RNA virus, for incorporation.
Because of this, there is no DNA damage and the risk of cancer is thought to be lower. Ma
In addition, to avoid the possibility of cancer caused by the c-Myc gene, which is thought to be another cause.
Therefore, we use technology to create iPS cells by introducing genes that are not associated with cancer.
Purpose of iPS cell bank
In recent years, advances in regenerative medicine technology have led to the practical application of treatments using iPS cells. In particular, iPS cells created from an individual’s own cells can reduce the risk of rejection, and are therefore expected to make medical treatment safer. Currently, a technology called organoids* is being researched to create three-dimensional miniature organs in vitro from iPS cells, and the liver, large intestine mucosa, and blood vessels have almost been realized. Theoretically, it is possible to create organoids of almost any organ, including the pancreas and lungs, as well as the brain and nerves, but currently organs such as the liver, kidneys, pancreas, lungs, intestines, and brain have been created. In the future, it will be possible to treat diseases by transplanting the created organoids. Therefore, in preparation for the arrival of an era in which people can cure their own diseases with their own cells, we recommend that people generate and store their own iPS cells while they are young and not sick, in preparation for future unforeseen illnesses.
*: Organoids are collections of cells with structures and functions similar to actual organs and tissues. When cells that have the ability to differentiate into various types of cells, such as embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), are cultured under certain conditions, they transform into multiple types of cells. When these cells interact and come together spontaneously, they develop structures and functions similar to those of actual organs and tissues. This is an organoid.
As a comprehensive wellness clinic that combines regenerative medicine, preventive medicine, and aesthetic medicine, our clinic is a registered medical institution based on the Act on the Safety of Regenerative Medicine, etc., in order to provide cutting-edge medical care optimized for each patient. We are actively working to introduce advanced medical treatments such as iPS cells, which are covered by Type 1 regenerative medicine, etc., and we will provide a specialized medical treatment system and individualized treatment with consideration for privacy.
About iPS cell culture supernatant (exosomes)
We provide iPS exosomes, which are produced by purifying iPS cell culture supernatant. Proteome analysis (total amount of proteins contained) of iPS exosomes reveals more than 4,000 types of proteins, while only about 1,300 types of proteins are identified in conventional mesenchymal stem cell exosomes. This proves that it has many functions, but they can be categorized into anti-inflammatory effects, apoptosis inhibition, mitochondrial activation, cell proliferation, reprogramming, DNA repair, and DNA demethylation. This means that exosomes also contain a large amount of each type of microRNA. In particular, microRNAs involved in mitochondrial activation are expected to have the effect of energizing and rejuvenating cells. In addition, microRNAs related to the latter three, reprogramming, DNA repair, and DNA demethylation, are involved in iPS conversion (normalization) of cancer cells, so iPS exosomes have the potential for new cancer treatments.
A clinical case of alopecia treatment: Single 5ml administration of iPS exosomes
Service features
- Production of autologous iPS cells: Production of iPS cells from cells derived from the person's skin or blood
- iPS supernatant drip/injection: Use of supernatant obtained by culturing and purifying iPS cells.
- Custom-made cosmetics: It is possible to create various skin care and hair care products that are fully customized and contain your own iPS supernatant liquid.
Long-term storage system: Long-term storage at -196 degrees under strict quality control - Medical collaboration support: Provision of information and collaboration with specialists with a view to future medical use
- Reliable individualized care: We provide a support system optimized for each individual throughout the entire process.
- Providing autologous (cell bank subscribers only) or allogeneic iPS cell culture supernatant (exosomes): Enables treatment of various diseases and rejuvenation.
Future prospects
Clinical application of iPS cells to organ creation and regenerative medicine for intractable diseases has only just begun. In order to further develop this technology in the future, we will need to engage in joint research between our field and research facilities. In the future, we are planning to begin preparations for regenerative medicine, with a view to collaborating with the University of Tokyo, Yokohama City University, and Tokyo University of Science, which are at the forefront of iPS organoid research. Through this, we aim to further contribute to the realization of next-generation medicine using autologous iPS cells. In addition, we will continue research and development of allogeneic iPS cell exosomes by identifying and extracting disease-specific microRNAs, with a view to applying them to lifestyle-related diseases, degenerative diseases, and even cancer.
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[Contact information]
Saisei no Mori Clinic Roppongi(Forest of Regeneration Clinic Roppongi)
iPS Regeneration Treatment Center
M’s BILD 4A, 7-10-1 Roppongi, Minato-ku, Tokyo
TEL 03-3401-3141 FAX 03-3401-3142
E-mail:roppongi@forest-regen-clin.jpn.org
Price
| Autologous iPS service | Detail | Number of people | per person Price (tax included) |
|---|---|---|---|
| Basic package | • HLA type disease risk diagnosis • iPS cell culture cost • 10 years storage fee |
1 | ＄70,000 |
| 3 | ＄64,000 | ||
| 5 | ＄58,000 | ||
| Culture supernatant (exosome) drip/injection | • 1Shot (10mL) | ＄5,000 | |
| • ５Shot (10mL)＋Benefits１Shot（10mL） | ＄25,000 |
| Allogeneic iPS service | Detail | Price(including tax) |
|---|---|---|
| Culture supernatant (exosome) drip | • １Shot (10mL) | ＄7,000 |
| ・１Shot (10mL) Hybrid nano-liposome formation | ＄7,000 | |
| Culture supernatant (exosome) injection | • １Shot (3mL) | ＄1,800 |
| • １Shot (3mL) Hybrid nano-liposome formation | ＄2,300 | |
| • ５Shot (3mL x 5) | ＄7,000 | |
| • ５Shot (3mL x 5) Hybrid nano-liposome formation | ＄11,500 |
Hybrid nano-liposome formation
The permeability of supernatant liquid is increased by 10 times by hybrid nano-liposome formation (processing option) iPS cell culture supernatant is a “water-soluble” raw material. Originally, the particles are fine raw materials. However, by making it into liposomes, it is possible to further increase its permeability. The raw materials are processed while being protected using liposome technology at low temperatures.
